Dublin Core
Title
A validated HPLC–FLD method for the determination of mycotoxin levels in sun dried fermented cocoa beans: Effect of cola nut extract and powder
Creator
Vincent Owusu Kyei-Baffour, John Edem Kongor, George Anyebuno, Agnes Simpson Budu, Saalia Kwesi Firibu, Emmanuel Ohene Afoakwa
Description
This study investigated mycotoxin concentrations in fermented sun-dried cocoa beans as affected by cola nut powder and cola nut extract using a 2 × 4 full factorial experimental design with treatment type (cola nut powder; cola nut extract) and treatment levels (0 g/5 kg, 50 g/5 kg, 100 g/5 kg and 150 g/5 kg) as principal factors. Aflatoxins B1, B2, G1, G2 and ochratoxin A (OTA) levels were studied using a validated HPLC – FLD analytical method. There was significant reduction in OTA (p < 0.05) from 8.32 μg/kg to 2.85 μg/kg and 3.02 μg/kg, respectively for the 150 g/5 kg cola nut extract and powder concentrations. The limit of quantification (LOQ) for the instrument was estimated as 0.60 μg/kg, 0.50 μg/kg, 0.78 μg/kg, 1.09 μg/kg and 2.30 μg/kg for aflatoxins B1, B2, G1, G2 and ochratoxin A, respectively. Aflatoxins (B1, B2, G1 and G2) levels for all treatments were below the limit of detection (LOD). The LOD was …
Publisher
Academic Press
Date
2021
Source
https://scholar.google.com/citations?view_op=view_citation&hl=en&user=EZuX1N8AAAAJ&cstart=100&pagesize=100&citation_for_view=EZuX1N8AAAAJ:XAEgxC7iu0MC
Language
English